Lysotracker and lysosensor staining
Web18 sept. 2013 · The lysosomal number, alkalinity, permeability and cathepsin B activity were determined by flow cytometry with ectodermal dysplasia-1, lysosensor and acridine orange staining, and using cathepsin B specific substrate, respectively. Results: Confocal imaging revealed that IONPs were markedly engulfed by microglia.
Lysotracker and lysosensor staining
Did you know?
Web2 iun. 2024 · As the key organelle for cellular degradation, lysosomes exhibit age-related changes such as increased size, number and content; increases and decreases in lysosomal hydrolase activity have also been reported ( Truschel et al., 2024; Sarkis et al., 1988; Cuervo and Dice, 1998; Hayflick, 1980; Bolanowski et al., 1983; Yoon et al., 2010; … Web10 ian. 2014 · Interestingly, this staining correlates with the location of Ca 2+ stores in human sperm cells and also with the observed Ned-19 fluorescence, associating NAADP’s binding site to the Ca 2+ stores. Sperm preincubation with Bafilomycin diminished Lysotracker Red and LysoSensor staining, confirming the acidic nature of these …
WebLysoTracker® and containing • Desiccate LysoSensor™ dyes When stored as 50 μL 1 mM stock • Protect from light directed, products solution in • Avoid freeze-thaw cycles 5 … WebAccording to Invitrogen Lysotracker is not suitable for flow cytometry. To confirm our data we are staining fixed cells with a LAMP1 antibody to compare the results of living cells …
WebWe have developed a Lysotracker staining assay that measures the enlarged lysosomes in patient-derived cells using both fluorescence intensity readout and fluorescence microscopic measurement. This phenotypic assay has been tested in patient cells obtained from several lysosomal storage diseases and validated using a known compound, methyl … Web2 sept. 2014 · LysoTracker is an acidotropic dye that stains cellular acidic compartments, including lysosomes and autolysosomes. LysoTracker has been used to detect …
WebTable 12.3 Summary of our LysoTracker and LysoSensor probes. * Absorption (Abs) and fluorescence emission (Em) maxima, determined in aqueous buffer or methanol; values may vary somewhat in cellular environments. † At pH 3. ‡ At pH 7; this dye has pH-dependent dual-excitation and dual-emission peaks. ND = Not determined.
WebLysoTracker products provide fluorescence detection for live-cell staining of acidic environments and can be used for labeling and tracing acidic organelles such as … quotes that prove romeo is impulsiveWebLysoSensor®系列探针是主要对活细胞中的酸性区室（如溶酶体、顶体精子）动态合成和功能进行研究的一类荧光探针，该类探针的染色具有向酸性，可通过质子化作用在酸性细 … quotes that prove odysseus is a heroWeb9 mar. 2015 · LysoTracker® and LysoSensor™ dyes 20 vials, each containing 50 μL 1 mM stock solution in anhydrous DMSO • ≤–20°C • Desiccate • Protect from light • Avoid freeze-thaw cycles • Do not store in a frost-free freezer • Store in single-use aliquots, if … shirts you can designWebIf you plan to do live-cell-imaging, Tf and lysotracker would work fine. Just be aware that Tf will stain the cell surface first, then endosomes (after about 5-10 min), and may also traffick... quotes that prove hamlet is not crazyWebLysoTracker staining has been commonly used for exam- ... significant increase of both LysoTracker and LysoSensor staining was observed in cells treated with EBSS, PP242 quotes that prove hamlet is madWeb12 mar. 2024 · The LysoTracker probes (Invitrogen/Molecular Probes) are used to label lysosomes in live cells, as described in this protocol. They are more selective for acidic organelles than the classically used neutral red or acridine orange dyes. They are freely membrane permeant at neutral pH and effectively label lysosomes at nanomolar … shirts you don\u0027t tuck inWeb17 sept. 2024 · Lysosensor or Lysotracker (similar probes with different spectral ranges) are quite straightforward to use. They are usually added to media and are taken up by … shirts you can wear without a bra